Nanobiotechnological applications in the medical area belong to the most advanced and promising fields. As the field of nanobiotechnology is rather young, a comprehensive picture of the state-of-the-art of research and commercial nanobiotechnology activities in Europe is still missing. Furthermore, to enable the exploitation of this technology and a proactive approach to increase European competitiveness, information is needed on future directions of nanobiotechnology applications and on possible drivers and barriers for its development.